Building a BLAST database with local sequences has never been this easy. Follow the instructions below and create your own searchable BLAST db in seconds.
This is for creating custom database for BLAST
Let me know if you have any questions.
Usage:
makeblastdb -in database_file -parse_seqids -hash_index -taxid 99999
Example:
makeblastdb -in nr -parse_seqids -hash_index -taxid 3694
Output:
It will search nr database and get all Populus trichocarpa related genes
That’s all falks!